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primary antibody against p ir β  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary antibody against p ir β
    Key materials and software.
    Primary Antibody Against P Ir β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+ir+%CE%B2+primary+antibody/Phospho-IRS-1+(Ser307)+Antibody/pmc11914097-17-0-5
    Average 96 stars, based on 494 article reviews
    primary antibody against p ir β - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders"

    Article Title: A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders

    Journal: Cell Discovery

    doi: 10.1038/s41421-025-00789-x

    Key materials and software.
    Figure Legend Snippet: Key materials and software.

    Techniques Used: Software, Recombinant, Luciferase, Reporter Gene Assay, Plasmid Preparation, Control, Transfection, Enzyme-linked Immunosorbent Assay, Staining

    Related Articles

    Lysis:

    Article Title: Subetta increases phosphorylation of insulin receptor β-subunit alone and in the presence of insulin
    Article Snippet: .. Preadipocyte medium (catalog number PM-1), adipocyte differentiation medium (catalog number DM-2), adipocyte maintenance medium (catalog number AM-1) and adipocyte basal medium (catalog number BM-1) were provided by Zen-Bio Inc. Lysis buffer (Tris, pH 7.4, NP-40, NaCl, protease and phosphatase inhibitors) was the product of Zen-Bio Inc. IR β-subunit primary antibody (catalog number 3025), phospho-IR-β primary antibody (catalog number 3026) and α-rabbit immunoglobulin G coupled to horseradish peroxidase secondary antibody (catalog number 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. NuPage (4–12%) Bis-Tris polyacrylamide gels (catalog number NP0323Box), MOPS buffer (3-( N -morpholino)propanesulfonic acid; catalog number NP0001), SeeBlue Molecular Weight Markers (catalog number LC5700), nitrocellulose membrane (catalog number LC2001), Transfer buffer (catalog number NP0006) and NuPage LDS sample buffer (catalog number NP0007) were purchased from Invitrogen (Carlsbad, CA, USA).



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    Image Search Results


    Key materials and software.

    Journal: Cell Discovery

    Article Title: A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders

    doi: 10.1038/s41421-025-00789-x

    Figure Lengend Snippet: Key materials and software.

    Article Snippet: Primary antibody against p-IR-β , Cell Signaling Technology, Inc., Shanghai, China , #2381.

    Techniques: Software, Recombinant, Luciferase, Reporter Gene Assay, Plasmid Preparation, Control, Transfection, Enzyme-linked Immunosorbent Assay, Staining

    Primer sequences used in real-time PCR quantification of mRNA

    Journal: Journal of Ginseng Research

    Article Title: The non-saponin fraction of Korean Red Ginseng (KGC05P0) decreases glucose uptake and transport in vitro and modulates glucose production via down-regulation of the PI3K/AKT pathway in vivo

    doi: 10.1016/j.jgr.2019.12.004

    Figure Lengend Snippet: Primer sequences used in real-time PCR quantification of mRNA

    Article Snippet: After washing, the membranes were probed overnight at 4°C with primary antibodies against β-actin, IRS-1, P-IRS-1, AKT, phospho-AKT, PI3K, and phospho-PI3K (Cell Signaling Technology, 1:1000).

    Techniques: Real-time Polymerase Chain Reaction

    Effects of KGC05P0 on basal and insulin action on gluconeogenesis pathway-related mRNA levels in HepG2 cells

    Journal: Journal of Ginseng Research

    Article Title: The non-saponin fraction of Korean Red Ginseng (KGC05P0) decreases glucose uptake and transport in vitro and modulates glucose production via down-regulation of the PI3K/AKT pathway in vivo

    doi: 10.1016/j.jgr.2019.12.004

    Figure Lengend Snippet: Effects of KGC05P0 on basal and insulin action on gluconeogenesis pathway-related mRNA levels in HepG2 cells

    Article Snippet: After washing, the membranes were probed overnight at 4°C with primary antibodies against β-actin, IRS-1, P-IRS-1, AKT, phospho-AKT, PI3K, and phospho-PI3K (Cell Signaling Technology, 1:1000).

    Techniques:

    Effects of KGC05P0 on protein and mRNA expression in liver from C57BL/6J and C57BLKS/J db/db mice. (A) Protein expressions, (B) mRNA expression of IRS-1, (C) the ratio of phosphorylated/total PI3K protein expression, (D) the ratio of phosphorylated/total AKT protein expression, (E) FoxO1 mRNA expression, (F) G6Pase mRNA expression, (G) PEPCK mRNA expression, (H) GLUT2 mRNA expression, (I) glucokinase mRNA expression, (J) phosphofructokinase mRNA expression, and (K) acetyl-CoA carboxylase mRNA expressions. Values are presented as mean ± SD, and different alphabets indicate significance at P < 0.05. NC, normal control; C, control; PC, positive control (inulin, 400 mg/kg b.w.); L, low (KGC05P0 100 mg/kg b.w.); M, medium (KGC05P0 200 mg/kg b.w.); H, high (KGC05P0 400 mg/kg b.w.).

    Journal: Journal of Ginseng Research

    Article Title: The non-saponin fraction of Korean Red Ginseng (KGC05P0) decreases glucose uptake and transport in vitro and modulates glucose production via down-regulation of the PI3K/AKT pathway in vivo

    doi: 10.1016/j.jgr.2019.12.004

    Figure Lengend Snippet: Effects of KGC05P0 on protein and mRNA expression in liver from C57BL/6J and C57BLKS/J db/db mice. (A) Protein expressions, (B) mRNA expression of IRS-1, (C) the ratio of phosphorylated/total PI3K protein expression, (D) the ratio of phosphorylated/total AKT protein expression, (E) FoxO1 mRNA expression, (F) G6Pase mRNA expression, (G) PEPCK mRNA expression, (H) GLUT2 mRNA expression, (I) glucokinase mRNA expression, (J) phosphofructokinase mRNA expression, and (K) acetyl-CoA carboxylase mRNA expressions. Values are presented as mean ± SD, and different alphabets indicate significance at P < 0.05. NC, normal control; C, control; PC, positive control (inulin, 400 mg/kg b.w.); L, low (KGC05P0 100 mg/kg b.w.); M, medium (KGC05P0 200 mg/kg b.w.); H, high (KGC05P0 400 mg/kg b.w.).

    Article Snippet: After washing, the membranes were probed overnight at 4°C with primary antibodies against β-actin, IRS-1, P-IRS-1, AKT, phospho-AKT, PI3K, and phospho-PI3K (Cell Signaling Technology, 1:1000).

    Techniques: Expressing, Control, Positive Control